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tegument proteins pp65  (Novus Biologicals)


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    Novus Biologicals tegument proteins pp65
    Roles of the autophagy machinery in different steps of the viral cycle. ( A ) Quantification of HCMV entry by immunofluorescence detection of the viral tegument protein <t>pp65</t> 2 h after contact with the virus (MOI 1). DAPI was used to stain nuclei. Percentages of pp65-positive cells correspond to viral entry into the indicated cell lines compared to control cells (deficient: Autophagy-deficient). ( B ) Quantification of HCMV genome replication 4 dpi at MOI 0.5 by real time PCR in the indicated cell lines, compared to control cells. ( C ) Expression of several viral proteins after HCMV infection of control and autophagy-deficient cell lines at MOI 0.5 for 1, 2 or 5 days post infection (dpi). Actin was used as a loading control. Full-length blots are presented in Fig. . ( D ) Indicated control and deficient cells were infected at MOI 0.5 with HCMV AD169 strain. Cell-associated viruses were collected 4 dpi and the amount of infectious virus was measured. Results represent the mean values of 3 independent experiments. ns: non-significant (Student’s t test).
    Tegument Proteins Pp65, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tegument+proteins+pp65/pmc06418312-251-21-24?v=Novus+Biologicals
    Average 90 stars, based on 1 article reviews
    tegument proteins pp65 - by Bioz Stars, 2026-07
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    1) Product Images from "Human cytomegalovirus hijacks the autophagic machinery and LC3 homologs in order to optimize cytoplasmic envelopment of mature infectious particles"

    Article Title: Human cytomegalovirus hijacks the autophagic machinery and LC3 homologs in order to optimize cytoplasmic envelopment of mature infectious particles

    Journal: Scientific Reports

    doi: 10.1038/s41598-019-41029-z

    Roles of the autophagy machinery in different steps of the viral cycle. ( A ) Quantification of HCMV entry by immunofluorescence detection of the viral tegument protein pp65 2 h after contact with the virus (MOI 1). DAPI was used to stain nuclei. Percentages of pp65-positive cells correspond to viral entry into the indicated cell lines compared to control cells (deficient: Autophagy-deficient). ( B ) Quantification of HCMV genome replication 4 dpi at MOI 0.5 by real time PCR in the indicated cell lines, compared to control cells. ( C ) Expression of several viral proteins after HCMV infection of control and autophagy-deficient cell lines at MOI 0.5 for 1, 2 or 5 days post infection (dpi). Actin was used as a loading control. Full-length blots are presented in Fig. . ( D ) Indicated control and deficient cells were infected at MOI 0.5 with HCMV AD169 strain. Cell-associated viruses were collected 4 dpi and the amount of infectious virus was measured. Results represent the mean values of 3 independent experiments. ns: non-significant (Student’s t test).
    Figure Legend Snippet: Roles of the autophagy machinery in different steps of the viral cycle. ( A ) Quantification of HCMV entry by immunofluorescence detection of the viral tegument protein pp65 2 h after contact with the virus (MOI 1). DAPI was used to stain nuclei. Percentages of pp65-positive cells correspond to viral entry into the indicated cell lines compared to control cells (deficient: Autophagy-deficient). ( B ) Quantification of HCMV genome replication 4 dpi at MOI 0.5 by real time PCR in the indicated cell lines, compared to control cells. ( C ) Expression of several viral proteins after HCMV infection of control and autophagy-deficient cell lines at MOI 0.5 for 1, 2 or 5 days post infection (dpi). Actin was used as a loading control. Full-length blots are presented in Fig. . ( D ) Indicated control and deficient cells were infected at MOI 0.5 with HCMV AD169 strain. Cell-associated viruses were collected 4 dpi and the amount of infectious virus was measured. Results represent the mean values of 3 independent experiments. ns: non-significant (Student’s t test).

    Techniques Used: Immunofluorescence, Virus, Staining, Control, Real-time Polymerase Chain Reaction, Expressing, Infection



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    Roles of the autophagy machinery in different steps of the viral cycle. ( A ) Quantification of HCMV entry by immunofluorescence detection of the viral tegument protein <t>pp65</t> 2 h after contact with the virus (MOI 1). DAPI was used to stain nuclei. Percentages of pp65-positive cells correspond to viral entry into the indicated cell lines compared to control cells (deficient: Autophagy-deficient). ( B ) Quantification of HCMV genome replication 4 dpi at MOI 0.5 by real time PCR in the indicated cell lines, compared to control cells. ( C ) Expression of several viral proteins after HCMV infection of control and autophagy-deficient cell lines at MOI 0.5 for 1, 2 or 5 days post infection (dpi). Actin was used as a loading control. Full-length blots are presented in Fig. . ( D ) Indicated control and deficient cells were infected at MOI 0.5 with HCMV AD169 strain. Cell-associated viruses were collected 4 dpi and the amount of infectious virus was measured. Results represent the mean values of 3 independent experiments. ns: non-significant (Student’s t test).
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    Image Search Results


    Roles of the autophagy machinery in different steps of the viral cycle. ( A ) Quantification of HCMV entry by immunofluorescence detection of the viral tegument protein pp65 2 h after contact with the virus (MOI 1). DAPI was used to stain nuclei. Percentages of pp65-positive cells correspond to viral entry into the indicated cell lines compared to control cells (deficient: Autophagy-deficient). ( B ) Quantification of HCMV genome replication 4 dpi at MOI 0.5 by real time PCR in the indicated cell lines, compared to control cells. ( C ) Expression of several viral proteins after HCMV infection of control and autophagy-deficient cell lines at MOI 0.5 for 1, 2 or 5 days post infection (dpi). Actin was used as a loading control. Full-length blots are presented in Fig. . ( D ) Indicated control and deficient cells were infected at MOI 0.5 with HCMV AD169 strain. Cell-associated viruses were collected 4 dpi and the amount of infectious virus was measured. Results represent the mean values of 3 independent experiments. ns: non-significant (Student’s t test).

    Journal: Scientific Reports

    Article Title: Human cytomegalovirus hijacks the autophagic machinery and LC3 homologs in order to optimize cytoplasmic envelopment of mature infectious particles

    doi: 10.1038/s41598-019-41029-z

    Figure Lengend Snippet: Roles of the autophagy machinery in different steps of the viral cycle. ( A ) Quantification of HCMV entry by immunofluorescence detection of the viral tegument protein pp65 2 h after contact with the virus (MOI 1). DAPI was used to stain nuclei. Percentages of pp65-positive cells correspond to viral entry into the indicated cell lines compared to control cells (deficient: Autophagy-deficient). ( B ) Quantification of HCMV genome replication 4 dpi at MOI 0.5 by real time PCR in the indicated cell lines, compared to control cells. ( C ) Expression of several viral proteins after HCMV infection of control and autophagy-deficient cell lines at MOI 0.5 for 1, 2 or 5 days post infection (dpi). Actin was used as a loading control. Full-length blots are presented in Fig. . ( D ) Indicated control and deficient cells were infected at MOI 0.5 with HCMV AD169 strain. Cell-associated viruses were collected 4 dpi and the amount of infectious virus was measured. Results represent the mean values of 3 independent experiments. ns: non-significant (Student’s t test).

    Article Snippet: To detect HCMV-infected cells, we used mouse monoclonal antibodies directed against the viral proteins IE1 and IE2 (clone E13; Biomerieux, 11–003), tegument proteins pp65 (Novus, B051M) and pp28 (Santa Cruz, clone CH19, sc-69749).

    Techniques: Immunofluorescence, Virus, Staining, Control, Real-time Polymerase Chain Reaction, Expressing, Infection

    Detection of HCMV-IE and pp65 in HGS ovarian carcinoma tissue sections. HCMV-IE and pp65 were detected frequently at different levels in tumor cells and in the stroma in HGS ovarian cancer tissue sections obtained at DEBPC and IDS after NACT. DEBPC = diagnostic excisional biopsy prechemotherapy, HCMV-IE = human cytomegalovirus immediate-early protein, HCMV-pp65 = human cytomegalovirus tegument protein, HGS = high grade serous ovarian carcinoma, IDS = interval debulking surgery, NACT = neoadjuvant chemotherapy.

    Journal: Medicine

    Article Title: Human cytomegalovirus in high grade serous ovarian cancer possible implications for patients survival

    doi: 10.1097/MD.0000000000009685

    Figure Lengend Snippet: Detection of HCMV-IE and pp65 in HGS ovarian carcinoma tissue sections. HCMV-IE and pp65 were detected frequently at different levels in tumor cells and in the stroma in HGS ovarian cancer tissue sections obtained at DEBPC and IDS after NACT. DEBPC = diagnostic excisional biopsy prechemotherapy, HCMV-IE = human cytomegalovirus immediate-early protein, HCMV-pp65 = human cytomegalovirus tegument protein, HGS = high grade serous ovarian carcinoma, IDS = interval debulking surgery, NACT = neoadjuvant chemotherapy.

    Article Snippet: Monoclonal antibodies against HCMV immediate-early protein (HCMV-IE) (Chemicon International, MA) and HCMV tegument protein pp65 (BioGenex, CA) were used for the detection of different HCMV proteins and antibodies against keratin 20 (Chemicon) or omitting primary antibodies were used as controls.

    Techniques: Diagnostic Assay